The influence of genotype and media on callus induction and green plant regeneration of F1 genotypes of Oryza sativa L.

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M.Z. Abdullah, G. Hamidah and A. Latifah

Abstract

Callus induction and green plant regeneration from cultured anthers of 33 F1 genotypes and rice variety, Puteh Perak were compared. Modified N6 (control) media namely G-1 and FJ have improved callus induction efficiency. Among the callus induction media, FJ was the best (5.33%), followed by N6 (2.69%) and G-1 (1.71%). For plant regeneration, modified MS media showed improvement, 24.11% for M-3 and 22.29% for M-2 as compared to N19 which was 14.15%. Similarly, for green plant regeneration, the responses were relatively superior in the modified media namely, M-3 (3.60%), M-1 (2.93%) and M-2 (2.31%) as compared to the control (N19) which recorded a 2.23% of green plant regeneration. Better green plant regeneration was obtained from callus induced in G-1(3.8%), followed by N6 (2.75%) and FJ (2.57%). In this study, differential genotype and media responses were clearly indicated in callus induction, total plant regeneration and green plant regeneration.

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