In vitro regeneration of Fragaria vesca L.

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A.C.K. Ling and A. Wetten

Abstract
Fragaria vesca L., a diploid relative of the cultivated octoploid strawberry Fragaria x ananassa, consists of seasonal Fragaria vesca f. vesca (F. vesca) and everbearing Fragaria vesca f. semperflorens (F. v. semperflorens). Study on F. vesca L. showed that F. v. semperflorens had higher in vitro regeneration capabilities than F. vesca although they belong to the same genus and species in the hierarchical rank. F. vesca explants and F. v. semperflorens could not regenerate from either leaf or petiole segments on media without plant growth regulators. Plantlet regeneration from leaf explants was highest on media supplemented with 1 mg/litre 6-benzylaminopurine (BAP) in comparison with those cultured on media supplemented with 2, 3 and 4 mg/litre BAP. Plantlet regeneration from F. v. semperflorens petiole explants was best achieved at 0.1 mg/litre indole-3-butyric acid compared to 0, 0.2 and 0.4 mg/litre.

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